Molecular Biology-RA/SRA(Multiple Positions)

Qualification: M.Sc. with 2-5 Yrs Experience (Molecular Biology/ Strain Engineering)

Profile: Synthetic biology, Molecular biologist, Pathway engineering, Genetic engineering, Fungal genetics, Clone development

Primary competence:

·       Execution of projects related to genetic engineering, primarily involving cloning and protein expression in hosts like E. coli, Bacillus subtilis, Yarrowia lipolytica, Saccharomyces, P. pastoris, Trichoderma, and Aspergillus.

·       Responsible for the design and execution of R&D projects involving synthetic biology.

·       The position requires full-time bench work, performing cloning and expression-related experiments.

·       Hands-on experience in techniques such as end-point PCR, AGE, SDS-PAGE, RT-PCR, HCP assays, and other bioanalytical tools is essential.

·       Experience in pathway engineering through gene overexpression, knockout studies, stable genomic integration, upregulation, and downregulation.

·       Well-versed in molecular techniques including Gibson assembly and CRISPR-Cas9.

·       Day-to-day responsibilities include conducting molecular biology experiments such as PCR, plasmid isolation, gel electrophoresis (horizontal and vertical), western blotting, bioanalytical techniques, and microbial culture and maintenance.

·       Responsible for analysis, documentation, and reporting of experimental data.

Secondary competence:

·       Ensuring all project-related documentation, including reports, presentations, and team compliance, is in place. Also responsible for training team members on SOPs, analytical procedures, and data recording.

·       Strong analytical and reasoning skills.

·       Ability to work independently as well as collaboratively within a team.

Important: Candidates with molecular diagnostics experience are not eligible for this role.